陕西省犊牛安氏隐孢子虫多位点序列分型研究

doi:10.11843/j.issn.0366-6964.2017.10.020

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (10): 1969-1975.doi: 10.11843/j.issn.0366-6964.2017.10.020

陕西省犊牛安氏隐孢子虫多位点序列分型研究

任冠静, 王雪婷, 张会军, 宋军科*, 赵光辉*

西北农林科技大学动物医学院, 杨凌 712100

收稿日期:2017-04-28 出版日期:2017-10-23 发布日期:2017-10-23
通讯作者: 宋军科,讲师,主要从事人兽共患寄生虫病的防控研究,E-mail:sjk7998@163.com;赵光辉,副教授,主要从事人兽共患寄生虫病的防控研究,E-mail:zgh083@nwsuaf.edu.cn
作者简介:任冠静(1992-),女,河南禹州人,硕士生,主要从事分子病原学方面的研究,E-mail:guanjing0406@163.com
基金资助:
国家重点研发计划（2017YFD0501300）；国家自然科学基金面上项目（31572509）；陕西省科学技术研究发展规划资助项目（2016NY-113）；中国博士后面上项目（2016M592848）；陕西省博士后科研项目资助特别资助。

Multilocus Sequence Typing of Cryptosporidium andersoni from Calves in Shaanxi Province

REN Guan-jing, WANG Xue-ting, ZHANG Hui-jun, SONG Jun-ke*, ZHAO Guang-hui*

College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China

Received:2017-04-28 Online:2017-10-23 Published:2017-10-23

摘要/Abstract

摘要：

为了对陕西省犊牛安氏隐孢子虫（Cryptosporidium andersoni）进行多位点序列分型（multilocus sequence typing， MLST），本研究基于4个基因位点对陕西省4个地区的36份犊牛（0～6月龄）C.andersoni阳性粪便样品进行PCR检测和序列分析。结果显示，15份C.andersoni分离株在CM-MS1、CM-MS2、CM-MS3、CM-MS16 4个基因位点均被成功分型，形成A4、A4、A2、A1，A4、A4、A4、A1，A5、A4、A4、A1和A1、A4、A4、A1共4个MLST亚型。其中A4、A4、A4、A1广泛分布于各个养殖场与不同品种犊牛中，为该地区犊牛C.andersoni的优势亚型。该研究结果表明，陕西地区犊牛C.andersoni存在遗传多样性，具有多种MLST亚型。

Abstract:

To study the multilocus sequence types of Cryptosporidium andersoni from calves in Shaanxi Province, 36 fecal samples positive for C. andersoni were collected from 0-6 months calves in four regions of Shaanxi province and examined by the polymerase chain reaction (PCR). Sequence analysis was based on four genetic loci, namely CM-MS1, CM-MS2, CM-MS3 and CM-MS16. The results showed that a total of 15 C. andersoni isolates were successfully genotyped in all four loci and divided into 4 subtypes of MLST, namely A4, A4, A2, A1; A4, A4, A4, A1; A5, A4, A4, A1; A1, A4, A4, A1. Among them, the MLST (A4, A4, A4, A1), as the dominant subtype, was widely distributed in various farms and different breeds of calves. These findings indicated that genetic variation was presented in C. andersoni of calves in Shaanxi province and various MLST subtypes were found in these calves.

中图分类号:

S852.723

任冠静, 王雪婷, 张会军, 宋军科, 赵光辉. 陕西省犊牛安氏隐孢子虫多位点序列分型研究[J]. 畜牧兽医学报, 2017, 48(10): 1969-1975.

REN Guan-jing, WANG Xue-ting, ZHANG Hui-jun, SONG Jun-ke, ZHAO Guang-hui. Multilocus Sequence Typing of Cryptosporidium andersoni from Calves in Shaanxi Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1969-1975.

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参考文献

[1] FAYER R. Taxonomy and species delimitation in Cryptosporidium[J]. Exp Parasitol, 2010, 124(1): 90-97.
[2] XIAO L H. Molecular epidemiology of cryptosporidiosis: an update[J]. Exp Parasitol, 2010, 124(1): 80-89.
[3] XIAO L H, FENG Y Y. Zoonotic cryptosporidiosis[J]. FEMS Immunol Med Microbiol, 2008, 52(3): 309-323.
[4] IMRE K, LOBO L M, MATOS O, et al. Molecular characterisation of Cryptosporidium isolates from pre-weaned calves in Romania: is there an actual risk of zoonotic infections?[J]. Vet Parasitol, 2011, 181(2-4): 321-324.
[5] WANG R J, MA G P, ZHAO J F, et al. Cryptosporidium andersoni is the predominant species in post-weaned and adult dairy cattle in China[J]. Parasitol Int, 2011, 60(1): 1-4.
[6] ESTEBAN E, ANDERSON B C. Cryptosporidium muris: prevalence, persistency, and detrimental effect on milk production in a drylot dairy[J]. J Dairy Sci, 1995, 78(5): 1068-1072.
[7] ANDERSON B C. Cryptosporidiosis in bovine and human health[J]. J Dairy Sci, 1998, 81(11): 3036-3041.
[8] PAUL S, CHANDRA D, TEWARI A K, et al. Prevalence of Cryptosporidium andersoni: a molecular epidemiological survey among cattle in India[J]. Vet Parasitol, 2009, 161(1-2): 31-35.
[9] QI M, WANG R J, JING B, et al. Prevalence and multilocus genotyping of Cryptosporidium andersoni in dairy cattle and He cattle in Xinjiang, China[J]. Infect Genet Evol, 2016, 44: 313-317.
[10] HUANG J Y, YUE D Y, QI M, et al. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Ningxia, northwestern China[J]. BMC Vet Res, 2014, 10: 292.
[11] QI M Z, FANG Y Q, WANG X T, et al. Molecular characterization of Cryptosporidium spp. in pre-weaned calves in Shaanxi Province, north-western China[J]. J Med Microbiol, 2015, 64(Pt 1): 111-116.
[12] LEONI F, AMAR C, NICHOLS G, et al. Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000[J]. J Med Microbiol, 2006, 55(Pt 6): 703-707.
[13] LIU H, SHEN Y J, YIN J H, et al. Prevalence and genetic characterization of Cryptosporidium, Enterocytozoon, Giardia and Cyclospora in diarrheal outpatients in China[J]. BMC Infect Dis, 2014, 14: 25.
[14] JIANG Y Y, REN J H, YUAN Z Y, et al. Cryptosporidium andersoni as a novel predominant Cryptosporidium species in outpatients with diarrhea in Jiangsu Province, China[J]. BMC Infect Dis, 2014, 14: 555.
[15] MCNABB S J, HENSEL D M, WELCH D F, et al. Comparison of sedimentation and flotation techniques for identification of Cryptosporidium sp. oocysts in a large outbreak of human diarrhea[J]. J Clin Microbiol, 1985, 22(4): 587-589.
[16] KAR S, GAWLOWSKA S, DAUGSCHIES A, et al. Quantitative comparison of different purification and detection methods for Cryptosporidium parvum oocysts[J]. Vet Parasitol, 2011, 177(3-4): 366-370.
[17] FALL A, THOMPSON R C A, HOBBS R P, et al. Morphology is not a reliable tool for delineating species within Cryptosporidium[J]. J Parasitol, 2003, 89(2): 399-402.
[18] DÍAZ P, HADFIELD S J, QUÍLEZ J, et al. Assessment of three methods for multilocus fragment typing of Cryptosporidium parvum from domestic ruminants in north west Spain[J]. Vet Parasitol, 2012, 186(3-4): 188-195.
[19] SULAIMAN I M, HIRA P R, ZHOU L, et al. Unique endemicity of cryptosporidiosis in children in Kuwait[J]. J Clin Microbiol, 2005, 43(6): 2805-2809.
[20] XIAO L H, ESCALANTE L, YANG C F, et al. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus[J]. Appl Environ Microbiol, 1999, 65(4): 1578-1583.
[21] XIAO L H, LIMOR J, MORGAN U M, et al. Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites[J]. Appl Environ Microbiol, 2000, 66(12): 5499-5502.
[22] KATO S, LINDERGARD G, MOHAMMED H O. Utility of the Cryptosporidium oocyst wall protein (COWP) gene in a nested PCR approach for detection infection in cattle[J]. Vet Parasitol, 2003, 111(2-3): 153-159.
[23] FENG Y Y, YANG W L, RYAN U, et al. Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni[J]. J Clin Microbiol, 2011, 49(1): 34-41.
[24] WANG R J, JIAN F C, ZHANG L X, et al. Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni[J]. PLoS One, 2012, 7(8): e43782.
[25] TYZZER E E. A sporozoan found in the peptic glands of the common mouse[J]. Exp Biol Med, 1907, 5(1): 12-13.
[26] ANDERSON B C. Experimental infection in mice of Cryptosporidium muris isolated from a camel[J]. J Protozool, 1991, 38(6): 16S-17S.
[27] LINDSAY D S, UPTON S J, OWENS D S, et al. Cryptosporidium andersoni n. sp. (Apicomple xa: Cryptosporiidae) from cattle, Bos taurus[J]. J Eukaryot Microbiol, 2000, 47(1): 91-95.
[28] SATOH M, HIKOSAKA K, SASAKI T, et al. Characteristics of a novel type of bovine Cryptosporidium andersoni[J]. Appl Environ Microbiol, 2003, 69(1): 691-692.
[29] ZHAO W, WANG R J, ZHANG W Z, et al. MLST subtypes and population genetic structure of Cryptosporidium andersoni from dairy cattle and beef cattle in northeastern China's Heilongjiang Province[J]. PLoS One, 2014, 9(7): e102006.
[30] ZHAO G H, REN W X, GAO M, et al. Genotyping Cryptosporidium andersoni in cattle in Shaanxi Province, Northwestern China[J]. PLoS One, 2013, 8(4): e60112.

陕西省犊牛安氏隐孢子虫多位点序列分型研究

Multilocus Sequence Typing of Cryptosporidium andersoni from Calves in Shaanxi Province

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